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Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
Uniform coverage and confident variant detection
Produce consistent, high-quality libraries for next generation sequencing (NGS) target enrichment workflows with the TrueAmp Library Prep Kit. Powered by the Twist TrueAmp polymerase and optimized for enzymatic fragmentation, this workflow delivers high conversion efficiency and low GC bias. These features give balanced coverage across challenging genomic regions, providing the accuracy needed for reliable variant characterization.
Reliable uniformity across challenging regions
Improve coverage consistency to reduce AT- and GC-related dropout. Ensure better representation of difficult genomic regions for more comprehensive sequencing results.
Efficient sample conversion
Maximize library yields from low-input, formalin-fixed, paraffin-embedded (FFPE), and variable-quality samples. Preserve precious starting material and minimize the need for reruns.
High-fidelity amplification
Minimize amplification-induced errors and bias with the Twist TrueAmp polymerase. Achieve accurate detection of low-frequency variants to increase confidence in every call.
Product data
The TrueAmp Library Prep Kit delivers higher pre-capture yield, consistent library sizes, and stronger mean target coverage from low input, degraded FFPE DNA, preserving library complexity and increasing reliable sequencing results from challenging samples.
Figure 1. Performance comparison of enriched libraries with low-input FFPE degraded samples (DIN <2.2) between TrueAmp Library Prep Kit and competitor K kit, demonstrating the optimal solution for challenging sample applications. (A) The Twist TrueAmp Library Prep Kit generates superior pre-capture library yield, indicative of high library construction and amplification efficiency. (B) The Twist TrueAmp Library Prep Kit shows higher library complexity when compared with the competitor’s kits. This allows for more unique DNA molecules that are sequenceable in the library, reducing sequencing costs. (C) Achieves higher coverage.
The Twist TrueAmp Library Prep Kit delivers improved coverage uniformity and fewer zero coverage regions. Reduced target dropout increases confidence that critical regions are consistently sequenced.
Figure 2. Performance comparison of enriched libraries with low-input FFPE degraded samples (DIN <2.2) between TrueAmp Library Prep Kit and competitor K kit, demonstrating the optimal solution for challenging sample applications. (A) Delivers excellent coverage uniformity, measured by a lower fold-80 base penalty. (B) Reduced regions with no coverage, measured by Percentage of Zero Coverage Targets.
The Twist TrueAmp Polymerase Mix delivers more uniform coverage across high- and low-GC regions, maintaining performance even with increased PCR cycling.
Figure 3. Normalized GC bias trace showing improved coverage of the Twist TrueAmp polymerase. Libraries were prepared with Twist TrueAmp Library Prep Kit and amplified with different polymerases and cycles.
Upper panel: Normalized coverage against GC window plots comparing polymerases at 3 cycles of PCR.
Lower panel: Normalized coverage against GC window plots comparing polymerases at 16 cycles of PCR.
The Twist TrueAmp Library Prep Kit delivers consistent DNA library fragment sizes and sufficient yield across a wide range of sample inputs, enabling mixed input workflows while maintaining uniform coverage and reproducible results at scale.
Figure 4. Reliable library size with Twist TrueAmp Library Prep Kit, even from ultra-low inputs.
500 ng, 100 ng, 50 ng, 10 ng, and 1 ng (gDNA) were fragmented at 32°C. 3, 5, 6, 8, 10, and 14 cycles of PCR were used for amplification, respectively. Samples have been performed in duplicates.
(A) Electropherograms of NGS libraries generated with the Twist TrueAmp Library Prep Kit.
(B) Concentration of libraries after amplification for various DNA inputs.
Twist TrueAmp Library Prep Kit delivers repeatable DNA library fragment sizes across a broad range of input amounts supporting confidence in sequencing results and downstream analysis.
Figure 5. Tunability of Twist TrueAmp Library Prep Kit.
(A) Five electropherograms of NGS libraries generated using differing fragmentation times. 50 ng of high-quality gDNA was fragmented for various times at 32°C. 6 cycles of PCR were utilized for amplification.
(B) Median insert size vs time. 50 ng of high-quality gDNA was fragmented for various times at 32°C. Amplification was performed using 6 cycles of PCR. Samples were captured using the Twist Exome 2.0 panel.
Video
High yield and coverage where samples are hardest
The TrueAmp Library Prep Kit delivers higher pre-capture yield, consistent library sizes, and stronger mean target coverage from low input, degraded FFPE DNA, preserving library complexity and increasing reliable sequencing results from challenging samples.
Figure 1. Performance comparison of enriched libraries with low-input FFPE degraded samples (DIN <2.2) between TrueAmp Library Prep Kit and competitor K kit, demonstrating the optimal solution for challenging sample applications. (A) The Twist TrueAmp Library Prep Kit generates superior pre-capture library yield, indicative of high library construction and amplification efficiency. (B) The Twist TrueAmp Library Prep Kit shows higher library complexity when compared with the competitor’s kits. This allows for more unique DNA molecules that are sequenceable in the library, reducing sequencing costs. (C) Achieves higher coverage.
Uniform coverage with fewer missed targets
The Twist TrueAmp Library Prep Kit delivers improved coverage uniformity and fewer zero coverage regions. Reduced target dropout increases confidence that critical regions are consistently sequenced.
Figure 2. Performance comparison of enriched libraries with low-input FFPE degraded samples (DIN <2.2) between TrueAmp Library Prep Kit and competitor K kit, demonstrating the optimal solution for challenging sample applications. (A) Delivers excellent coverage uniformity, measured by a lower fold-80 base penalty. (B) Reduced regions with no coverage, measured by Percentage of Zero Coverage Targets.
Improved coverage across GC extremes
The Twist TrueAmp Polymerase Mix delivers more uniform coverage across high- and low-GC regions, maintaining performance even with increased PCR cycling.
Figure 3. Normalized GC bias trace showing improved coverage of the Twist TrueAmp polymerase. Libraries were prepared with Twist TrueAmp Library Prep Kit and amplified with different polymerases and cycles. Upper panel: Normalized coverage against GC window plots comparing polymerases at 3 cycles of PCR. Lower panel: Normalized coverage against GC window plots comparing polymerases at 16 cycles of PCR.
Built for consistent fragment sizes across inputs and workflows
The Twist TrueAmp Library Prep Kit delivers consistent DNA library fragment sizes and sufficient yield across a wide range of sample inputs, enabling mixed input workflows while maintaining uniform coverage and reproducible results at scale.
Figure 4. Reliable library size with Twist TrueAmp Library Prep Kit, even from ultra-low inputs. 500 ng, 100 ng, 50 ng, 10 ng, and 1 ng (gDNA) were fragmented at 32°C. 3, 5, 6, 8, 10, and 14 cycles of PCR were used for amplification, respectively. Samples have been performed in duplicates. (A) Electropherograms of NGS libraries generated with the Twist TrueAmp Library Prep Kit. (B) Concentration of libraries after amplification for various DNA inputs.
Controlled insert sizes for tunable performance
Twist TrueAmp Library Prep Kit delivers repeatable DNA library fragment sizes across a broad range of input amounts supporting confidence in sequencing results and downstream analysis.
Figure 5. Tunability of Twist TrueAmp Library Prep Kit. (A) Five electropherograms of NGS libraries generated using differing fragmentation times. 50 ng of high-quality gDNA was fragmented for various times at 32°C. 6 cycles of PCR were utilized for amplification. (B) Median insert size vs time. 50 ng of high-quality gDNA was fragmented for various times at 32°C. Amplification was performed using 6 cycles of PCR. Samples were captured using the Twist Exome 2.0 panel.
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High yield and coverage where samples are hardest
The TrueAmp Library Prep Kit delivers higher pre-capture yield, consistent library sizes, and stronger mean target coverage from low input, degraded FFPE DNA, preserving library complexity and increasing reliable sequencing results from challenging samples.
Uniform coverage with fewer missed targets
The Twist TrueAmp Library Prep Kit delivers improved coverage uniformity and fewer zero coverage regions. Reduced target dropout increases confidence that critical regions are consistently sequenced.
Improved coverage across GC extremes
The Twist TrueAmp Polymerase Mix delivers more uniform coverage across high- and low-GC regions, maintaining performance even with increased PCR cycling.
Built for consistent fragment sizes across inputs and workflows
The Twist TrueAmp Library Prep Kit delivers consistent DNA library fragment sizes and sufficient yield across a wide range of sample inputs, enabling mixed input workflows while maintaining uniform coverage and reproducible results at scale.
Controlled insert sizes for tunable performance
Twist TrueAmp Library Prep Kit delivers repeatable DNA library fragment sizes across a broad range of input amounts supporting confidence in sequencing results and downstream analysis.
Built for More: Uniform Coverage, Higher Yields, and Confident Variant Characterization
How it works
